miR-17-5p promotes proliferation by targeting SOCS6 in gastric cancer cells

FEBS Lett. 2014 Jun 5;588(12):2055-62. doi: 10.1016/j.febslet.2014.04.036. Epub 2014 May 5.

Abstract

This study aimed to test the exact functions and potential mechanisms of miR-17-5p in gastric cancer. Using real-time PCR, miR-17-5p was found to be expressed more highly in gastric cancer compared with-normal tissues. Gain- and loss-of-function assays demonstrated that miR-17-5p increased the proliferation and growth of gastric cancer cells in vitro and in vivo. Through reporter gene and western blot assays, SOCS6 was shown to be a direct target of miR-17-5p, and proliferative assays confirmed that SOCS6 exerted opposing function to that of miR-17-5p in gastric cancer. In short, miR-17-5p might function as a pro-proliferative factor by repressing SOCS6 in gastric cancer.

Keywords: Gastric cancer; Proliferation; SOCS6; miR-17-92; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogenesis / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / genetics*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology*
  • Suppressor of Cytokine Signaling Proteins / genetics*

Substances

  • MIRN17 microRNA, human
  • MicroRNAs
  • SOCS6 protein, human
  • Suppressor of Cytokine Signaling Proteins