Expression levels of surfactant-associated proteins and inflammation cytokines in serum and bronchoalveolar lavage fluid among coal miners: a case-control study

J Occup Environ Med. 2014 May;56(5):484-8. doi: 10.1097/JOM.0000000000000169.

Abstract

Objective: To investigate whether the Th1/Th2 balance and expressions of surfactant-associated proteins and cytokines in serum and bronchoalveolar lavage fluid (BALF) are associated with the development of coal workers' pneumoconiosis (CWP).

Methods: A case-control study was conducted among 72 CWP cases and 68 controls. Th1 and Th2 populations were measured by flow cytometry. Expressions of surfactant-associated proteins A and D (SPA and SPD) and cytokines in serum and BALF were detected by enzyme-linked-immunosorbent serologic assay. Data were analyzed by t test and logistic regression.

Results: Higher Th2 and lower Th1/Th2 were observed in CWP (P < 0.05). Increased CWP risk was associated with elevated BALF-interleukin-10 (odds ratio [OR]: 25.97; 95% confidence interval [CI]: 2.17 to 49.77), serum-SPA/BALF-SPA (OR: 12.87; 95% CI: 1.11 to 148.72), and serum-SPD/BALF-SPD (OR: 77.91; 95% CI: 5.64 to 161.46), decreased BALF-SPA (OR: 0.06; 95% CI: 0.01 to 0.31) and BALF-SPD (OR: 0.07; 95% CI: 0.02 to 0.36).

Conclusions: The development of CWP might be associated with Th1/Th2 imbalance and increased BALF-interleukin-10, serum-SPA/BALF-SPA, and serum-SPD/BALF-SPD.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Air Pollutants, Occupational / adverse effects
  • Air Pollutants, Occupational / analysis
  • Anthracosis / etiology
  • Anthracosis / metabolism*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / immunology*
  • Case-Control Studies
  • Coal Mining*
  • Cytokines / blood*
  • Dust
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Humans
  • Inhalation Exposure / adverse effects
  • Middle Aged
  • Occupational Diseases / etiology
  • Occupational Diseases / metabolism*
  • Odds Ratio
  • Pulmonary Surfactant-Associated Protein A / blood
  • Pulmonary Surfactant-Associated Protein A / metabolism*
  • Pulmonary Surfactant-Associated Protein D / blood
  • Pulmonary Surfactant-Associated Protein D / metabolism*

Substances

  • Air Pollutants, Occupational
  • Cytokines
  • Dust
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Protein D