Human Tudor staphylococcal nuclease (Tudor-SN) protein modulates the kinetics of AGTR1-3'UTR granule formation

Xingjie Gao; Xuebin Shi; Xue Fu; Lin Ge; Yi Zhang; Chao Su; Xi Yang; Olli Silvennoinen; Zhi Yao; Jinyan He; Minxin Wei; Jie Yang

doi:10.1016/j.febslet.2014.04.045

Human Tudor staphylococcal nuclease (Tudor-SN) protein modulates the kinetics of AGTR1-3'UTR granule formation

FEBS Lett. 2014 Jun 13;588(13):2154-61. doi: 10.1016/j.febslet.2014.04.045. Epub 2014 May 8.

Authors

Xingjie Gao¹, Xuebin Shi², Xue Fu¹, Lin Ge², Yi Zhang¹, Chao Su¹, Xi Yang³, Olli Silvennoinen⁴, Zhi Yao², Jinyan He², Minxin Wei⁵, Jie Yang⁶

Affiliations

¹ Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, Tianjin 300070, China; Key Laboratory of Educational Ministry of China, Tianjin Medical University, Tianjin 300070, China.
² Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, Tianjin 300070, China; Key Laboratory of Educational Ministry of China, Tianjin Medical University, Tianjin 300070, China.
³ Department of Immunology, University of Manitoba, 471 Apotex Centre, 750 McDermot Avenue, Winnipeg R3E 0T5, Canada.
⁴ Institute of Medical Technology, University of Tampere, Tampere University Hospital, Biokatu 8, FI-33014 Tampere, Finland.
⁵ Department of Cardiovascular Surgery, Tianjin Medical University General Hospital, Tianjin 300070, China. Electronic address: [email protected].
⁶ Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, Tianjin 300070, China; Key Laboratory of Educational Ministry of China, Tianjin Medical University, Tianjin 300070, China. Electronic address: [email protected].

PMID: 24815690
DOI: 10.1016/j.febslet.2014.04.045

Abstract

Human Tudor staphylococcal nuclease (Tudor-SN) interacts with the G3BP protein and is recruited into stress granules (SGs), the main type of discrete RNA-containing cytoplasmic foci structure that is formed under stress conditions. Here, we further demonstrate that Tudor-SN binds and co-localizes with AGTR1-3'UTR (3'-untranslated region of angiotensin II receptor, type 1 mRNA) into SG. Tudor-SN plays an important role in the assembly of AGTR1-3'UTR granules. Moreover, endogenous Tudor-SN knockdown can decrease the recovery kinetics of AGTR1-3'UTR granules. Collectively, our data indicate that Tudor-SN modulates the kinetics of AGTR1-3'UTR granule formation, which provides an additional biological role of Tudor-SN in RNA metabolism during stress.

Keywords: 3′UTR; AGTR1; Stress granules; Tudor-SN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions* / genetics
Cytoplasmic Granules / genetics
Cytoplasmic Granules / metabolism
Endonucleases
Fluorescence Recovery After Photobleaching
Gene Knockdown Techniques
HeLa Cells
Humans
Kinetics
Nuclear Proteins / antagonists & inhibitors
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptor, Angiotensin, Type 1 / genetics*
Stress, Physiological

Substances

3' Untranslated Regions
AGTR1 protein, human
Nuclear Proteins
RNA, Messenger
Receptor, Angiotensin, Type 1
Endonucleases
SND1 protein, human