Visible light induced fast synthesis of protein-polymer conjugates: controllable polymerization and protein activity

Xin Li; Lei Wang; Gaojian Chen; David M Haddleton; Hong Chen

doi:10.1039/c4cc02277g

Visible light induced fast synthesis of protein-polymer conjugates: controllable polymerization and protein activity

Chem Commun (Camb). 2014 Jun 21;50(49):6506-8. doi: 10.1039/c4cc02277g. Epub 2014 May 12.

Authors

Xin Li¹, Lei Wang, Gaojian Chen, David M Haddleton, Hong Chen

Affiliation

¹ The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P. R. China. [email protected].

PMID: 24817595
DOI: 10.1039/c4cc02277g

Abstract

Herein visible light is used to induce RAFT polymerization from protein for preparing protein-polymer conjugates at ambient temperature. Polymerization is fast and can be conveniently controlled with irradiation time. By site-specific polymerization of NIPAm to protein, the protein activity is maintained and in certain cases it presents an efficient on-off-switchable property.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry
Immobilized Proteins / chemistry
Immobilized Proteins / metabolism
Inorganic Pyrophosphatase / chemistry
Inorganic Pyrophosphatase / metabolism
Light*
Polymerization
Polymers / chemistry*
Proteins / chemistry*
Proteins / metabolism
Temperature

Substances

Acrylic Resins
Immobilized Proteins
Polymers
Proteins
poly-N-isopropylacrylamide
Inorganic Pyrophosphatase