Abstract
The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzyme-recognition sites.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Assay
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Cadherins / genetics*
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CpG Islands / genetics*
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DNA Methylation*
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DNA Primers
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Deoxyguanine Nucleotides / chemistry*
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Fluorescein / chemistry*
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Fluorescence
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Humans
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Neoplasms / genetics*
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Polymerase Chain Reaction / methods*
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Promoter Regions, Genetic / genetics
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Tumor Cells, Cultured
Substances
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Cadherins
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DNA Primers
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Deoxyguanine Nucleotides
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deoxyguanosine triphosphate
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Fluorescein