Effect of highly mineralized natural water on redox processes in HaCaT keratinocytes

N S Zaitseva; A V Chechushkov; P M Kozhin; A E Lemza; V O Tkachev; M V Solomatina; V A Starostenko

doi:10.1007/s10517-014-2457-5

Effect of highly mineralized natural water on redox processes in HaCaT keratinocytes

Bull Exp Biol Med. 2014 Apr;156(6):810-2. doi: 10.1007/s10517-014-2457-5. Epub 2014 May 3.

Authors

N S Zaitseva¹, A V Chechushkov, P M Kozhin, A E Lemza, V O Tkachev, M V Solomatina, V A Starostenko

Affiliation

¹ Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, [email protected].

PMID: 24824704
DOI: 10.1007/s10517-014-2457-5

Abstract

We studied ROS production by HaCaT keratinocytes, the state of transmembrane mitochondrial potential, and activation of transcription factor Nrf2 in response to brine exposure. It was demonstrated that this exposure induces rapid but moderate decrease in mitochondrial potential, stimulates ROS production, and leads to activation of transcription factor Nrf2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / drug effects
Cell Line
Flow Cytometry
Fluorescent Antibody Technique
Humans
Keratinocytes / drug effects
Keratinocytes / metabolism*
Keratinocytes / physiology*
Membrane Potential, Mitochondrial / drug effects
Membrane Potential, Mitochondrial / physiology*
NF-E2-Related Factor 2 / metabolism*
Oxidation-Reduction
Reactive Oxygen Species / metabolism*
Salts / pharmacology*

Substances

NF-E2-Related Factor 2
NFE2L2 protein, human
Reactive Oxygen Species
Salts
brine