Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: the VetPath study

Vet Microbiol. 2014 Aug 6;172(1-2):202-15. doi: 10.1016/j.vetmic.2014.04.008. Epub 2014 Apr 21.

Abstract

VetPath is an ongoing pan-European antibiotic susceptibility monitoring programme collecting pathogens from diseased antimicrobial non-treated cattle, pigs and poultry. In the current study, 1001 isolates from cattle and pig respiratory tract infections were tested for their antimicrobial susceptibilities. Non-replicate lung samples or nasopharyngeal/nasal swabs were collected from animals with acute clinical signs in 11 countries during 2002-2006. Pasteurella multocida and Mannheimia haemolytica from cattle and P. multocida, Actinobacillus pleuropneumoniae and Streptococcus suis from pigs were isolated by standard methods. S. suis was also isolated from meningitis cases. MICs of 16 antibiotics were assessed centrally by broth microdilution following CLSI recommendations. Results were interpreted using CLSI breakpoints where available. P. multocida (231) and M. haemolytica (138) isolates were all susceptible to amoxicillin/clavulanic acid, ceftiofur, enrofloxacin and trimethoprim/sulfamethoxazole. Resistance to florfenicol and spectinomycin was 0.4% and 3.5% in P. multocida, respectively, and absent in M. haemolytica isolates. Tetracycline resistance was 5.7% and 14.6% for P. multocida and M. haemolytica. In pigs, 230 P. multocida, 220 A. pleuropneumoniae and 182 S. suis isolates were recovered. Resistance to amoxicillin/clavulanic acid, ceftiofur, enrofloxacin, florfenicol, tiamulin and tilmicosin was absent or <1%. Trimethoprim/sulfamethoxazole resistance was 3-6% and tetracycline resistance varied from 14.7% in A. pleuropneumoniae to 81.8% in S. suis. In conclusion, low resistance to antibiotics with defined clinical breakpoints, except for tetracycline, was observed among the major respiratory tract pathogens recovered from cattle and pigs. Since for approximately half of the antibiotics in this panel no CLSI-defined breakpoints were available, setting of the missing veterinary breakpoints is important.

Keywords: Antimicrobial resistance; Cattle; Minimal inhibitory concentrations; Pigs; Surveillance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacillus pleuropneumoniae / drug effects
  • Actinobacillus pleuropneumoniae / isolation & purification
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Cattle
  • Cattle Diseases / microbiology*
  • Drug Resistance, Bacterial*
  • Europe
  • Gram-Negative Bacterial Infections / microbiology
  • Gram-Negative Bacterial Infections / veterinary
  • Gram-Positive Bacterial Infections / microbiology
  • Gram-Positive Bacterial Infections / veterinary
  • Mannheimia haemolytica / drug effects
  • Mannheimia haemolytica / isolation & purification
  • Microbial Sensitivity Tests
  • Pasteurella multocida / drug effects
  • Pasteurella multocida / isolation & purification
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / veterinary*
  • Streptococcus suis / drug effects
  • Streptococcus suis / isolation & purification
  • Swine
  • Swine Diseases / microbiology*

Substances

  • Anti-Bacterial Agents