The unsaponifiable fraction of extra virgin olive oil promotes apoptosis and attenuates activation and homing properties of T cells from patients with inflammatory bowel disease

A Cárdeno; M K Magnusson; H Strid; C Alarcón de La Lastra; M Sánchez-Hidalgo; L Ohman

doi:10.1016/j.foodchem.2014.04.016

The unsaponifiable fraction of extra virgin olive oil promotes apoptosis and attenuates activation and homing properties of T cells from patients with inflammatory bowel disease

Food Chem. 2014 Oct 15:161:353-60. doi: 10.1016/j.foodchem.2014.04.016. Epub 2014 Apr 13.

Authors

A Cárdeno¹, M K Magnusson², H Strid³, C Alarcón de La Lastra⁴, M Sánchez-Hidalgo⁴, L Ohman⁵

Affiliations

¹ Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
² Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden.
³ Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden.
⁴ Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
⁵ Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden. Electronic address: [email protected].

PMID: 24837962
DOI: 10.1016/j.foodchem.2014.04.016

Abstract

The unsaponifiable fraction (UF) of extra virgin olive oil (EVOO) possesses anti-inflammatory properties and exerts preventative effects in murine models of inflammatory bowel disease (IBD). The present study was designed to determine the in vitro effects of UF on blood and intestinal T cells from IBD patients and healthy subjects. The T cell phenotype was investigated by flow cytometry and cytokine secretion was determined by ELISA. The presence of UF of EVOO promoted apoptosis and attenuated activation of intestinal and blood T cells isolated from IBD patients, decreasing the frequency of CD69(+) and CD25(+) T cells and, also, the secretion of IFN-γ. Moreover, UF reduced the expression of the gut homing receptor integrin β7 on blood T cells from IBD patients. In conclusion, UF modulates the activity and the gut homing capacity of T cells, and might therefore be considered as a dietary complement with an anti-inflammatory role in IBD patients.

Keywords: Extra virgin olive oil; Inflammatory bowel disease; T cells; Unsaponifiable fraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Animals
Anti-Inflammatory Agents / pharmacology
Apoptosis / drug effects*
Cells, Cultured
Female
Humans
Inflammatory Bowel Diseases / immunology*
Integrin beta Chains / analysis
Lymphocyte Activation / drug effects*
Male
Mice
Middle Aged
Olive Oil
Plant Oils / pharmacology*
T-Lymphocytes / drug effects*
T-Lymphocytes / immunology
T-Lymphocytes / physiology

Substances

Anti-Inflammatory Agents
Integrin beta Chains
Olive Oil
Plant Oils
integrin beta7