Rosuvastatin inhibits TIMP-2 and promotes myocardial angiogenesis

Pharmacology. 2014;93(3-4):178-84. doi: 10.1159/000360860. Epub 2014 May 17.

Abstract

Background: Angiogenesis is usually driven by inflammation. Matrix metalloproteinases MMP-3 and MMP-9 and tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 are implicated in vascular remodeling. TIMP-2 exhibits antiangiogenic properties. Statins show benefits that are additional to lipid lowering including pro- and antiangiogenic properties. Atherosclerotic lesions in the coronary arteries have been well studied, but less is known about the fine terminal branches of the myocardial vasculature.

Methods: To examine this, we studied rosuvastatin (RSV) treatment in ApoE knockout (ApoE(-/-)) mice fed a high cholesterol (HC) diet. Hearts from ApoE(-/-) mice on a normal diet, HC diet and HC diet with RSV were harvested to determine MMP-3, MMP-9, TIMP-1, TIMP-2, vascular endothelial growth factor (VEGF)-A and estrogen receptor-α (ER-α) mRNA.

Results: RSV inhibited TIMP-1 and TIMP-2 expression and enhanced myocardial VEGF-A and ER-α expression, independently of plasma lipid level changes, but had no effect on MMP-3 and MMP-9 expression.

Conclusions: These modulations of TIMPs, VEGF and ER-α expression induced by RSV may act as local stimulating factors for arteriolar growth in the myocardium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apolipoproteins E / genetics*
  • Cholesterol, Dietary / administration & dosage
  • Fluorobenzenes / pharmacology*
  • Gene Expression Regulation / drug effects
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology
  • Lipids / blood
  • Matrix Metalloproteinase 3 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neovascularization, Physiologic / drug effects*
  • Pyrimidines / pharmacology*
  • Rosuvastatin Calcium
  • Sulfonamides / pharmacology*
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism
  • Tissue Inhibitor of Metalloproteinase-2 / antagonists & inhibitors*
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Apolipoproteins E
  • Cholesterol, Dietary
  • Fluorobenzenes
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • Lipids
  • Pyrimidines
  • Sulfonamides
  • Timp1 protein, mouse
  • Tissue Inhibitor of Metalloproteinase-1
  • Vascular Endothelial Growth Factor A
  • Tissue Inhibitor of Metalloproteinase-2
  • Rosuvastatin Calcium
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 9