Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil

Franciéli P Rozales; Vanessa B Ribeiro; Cibele M Magagnin; Mariana Pagano; Larissa Lutz; Diego R Falci; Adão Machado; Afonso L Barth; Alexandre P Zavascki

doi:10.1016/j.ijid.2014.01.005

Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil

Int J Infect Dis. 2014 Aug:25:79-81. doi: 10.1016/j.ijid.2014.01.005. Epub 2014 May 21.

Authors

Franciéli P Rozales¹, Vanessa B Ribeiro¹, Cibele M Magagnin², Mariana Pagano¹, Larissa Lutz³, Diego R Falci⁴, Adão Machado⁵, Afonso L Barth¹, Alexandre P Zavascki⁶

Affiliations

¹ Programa de Pós Graduação em Ciências Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil.
² Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil; Programa de Pós Graduação em Ciências Médicas, Faculdade de Medicina, UFRGS, Porto Alegre, Brazil.
³ Unidade de Microbiologia, Serviço de Patologia Clínica, HCPA, Porto Alegre, Brazil.
⁴ Infection Control Service Hospital Nossa Senhora da Conceição, Porto Alegre, Brazil.
⁵ Infection Control Service Hospital Beneficiência Portuguesa, Porto Alegre, Brazil.
⁶ Infectious Diseases Service, Hospital de Clínicas de Porto, 2350 Ramiro Barcelos St, 90035-903, Porto Alegre, Brazil; Department of Internal Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: [email protected].

PMID: 24857802
DOI: 10.1016/j.ijid.2014.01.005

Abstract

Objectives: To evaluate the emergence of New Delhi metallo-β-lactamase 1 (NDM-1)-producing Enterobacteriaceae isolates in Brazil.

Methods: From April to October 2013, following the detection of the first NDM-1-producing isolate, a surveillance study was performed for the detection of blaNDM-1 among Enterobacteriaceae isolates with reduced susceptibility to carbapenems in 17 hospitals of Porto Alegre, Brazil. Real-time PCR was used to determine the presence of carbapenemase genes, which were further sequenced. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE).

Results: A total of 1134 isolates were evaluated. blaNDM-1 was detected in 11 (0.97%) isolates: nine Enterobacter cloacae complex (eight belonging to a single clone recovered from two distinct hospitals and the other strain from a third hospital) and two Morganella morganii (belonging to a single clone recovered from one hospital). Most isolates presented high-level resistance to carbapenems.

Conclusions: NDM-1-producing Enterobacteriaceae have emerged rapidly in the hospitals of the Brazilian city where they were first detected. The emergence of NDM-1 in Brazil is of great concern, since it is a severe threat to antimicrobial therapy against Enterobacteriaceae in this country.

Keywords: Carbapenemase; Epidemiology; NDM-1; Resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Brazil / epidemiology
Communicable Diseases, Emerging*
Cross Infection
Enterobacteriaceae / drug effects
Enterobacteriaceae / genetics*
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / epidemiology*
Enterobacteriaceae Infections / microbiology*
Humans
Microbial Sensitivity Tests
beta-Lactamases / biosynthesis
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
beta-Lactamases
beta-lactamase NDM-1