Functionalized polyglycerol amine nanogels as nanocarriers for DNA

Macromol Biosci. 2014 Sep;14(9):1215-21. doi: 10.1002/mabi.201400144. Epub 2014 May 26.

Abstract

Polyglycerol based nanogels (nPG) can function as cellular delivery systems. These nPGs are synthesized with different amine densities (nPG amines) by acid-catalyzed epoxide-opening polymerization using a mini-emulsion approach and surface modification. All the synthesized nanogels are characterized by NMR, dynamic light scattering, and ζ-potential, showing slightly positive surface charge and a homogeneous size of ≈100 nm. The use of these systems for delivery applications is demonstrated with regard to polyplex formation, cytotoxicity, and cellular uptake studies. It is depicted that the CE50 value of the high loaded nPG amines is eight times higher than the low loaded ones. The influence of the amine loading percentage on the nanogel and the effects of polyvalency in these architecture is discussed.

Keywords: DNA delivery; cationic architectures; cellular uptake; polyglycerol based nanogels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines* / chemical synthesis
  • Amines* / chemistry
  • Amines* / pharmacology
  • Animals
  • Cell Line
  • DNA* / chemistry
  • DNA* / pharmacology
  • Drug Carriers* / chemical synthesis
  • Drug Carriers* / chemistry
  • Drug Carriers* / pharmacology
  • Glycerol* / chemical synthesis
  • Glycerol* / chemistry
  • Glycerol* / pharmacology
  • Light
  • Magnetic Resonance Spectroscopy
  • Materials Testing*
  • Mice
  • Nanoparticles / chemistry*
  • Polymers* / chemical synthesis
  • Polymers* / chemistry
  • Polymers* / pharmacology
  • Scattering, Radiation

Substances

  • Amines
  • Drug Carriers
  • Polymers
  • polyglycerol
  • DNA
  • Glycerol