Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

Haibo Li; Hailong Wang; Dianshuai Huang; Lijia Liang; Yuejiao Gu; Chongyang Liang; Shuping Xu; Weiqing Xu

doi:10.1063/1.4878715

Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

Rev Sci Instrum. 2014 May;85(5):056109. doi: 10.1063/1.4878715.

Authors

Haibo Li¹, Hailong Wang¹, Dianshuai Huang², Lijia Liang¹, Yuejiao Gu¹, Chongyang Liang², Shuping Xu¹, Weiqing Xu¹

Affiliations

¹ State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People's Republic of China.
² Institute of Frontier Medical Science, Jilin University, Changchun 130021, People's Republic of China.

PMID: 24880434
DOI: 10.1063/1.4878715

Abstract

A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling techniques. Besides, nano-sized particles could be observed and located by dark field microscopes. Therefore, comparing with the commercial Raman spectrometers, much more researches based on Raman spectroscopy could be carried out on this integrated Raman platform, especially in the fields of analyzing biological tissues and subwavelength samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Fluorescence*
Humans
Optical Imaging* / instrumentation
Optical Imaging* / methods
Spectrum Analysis, Raman* / instrumentation
Spectrum Analysis, Raman* / methods