A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation

Chem Commun (Camb). 2014 Jul 18;50(56):7492-4. doi: 10.1039/c4cc02974g.

Abstract

We report here a novel fluorescent chemical probe which stains distinct neural stem/progenitor cells (NSPCs) by binding to acid ceramidase in mouse neurospheres. is distributed evenly or unevenly to the daughter cells during multiple mitoses enabling the live imaging of symmetric and asymmetric divisions of isolated NSPCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asymmetric Cell Division* / physiology
  • Cells, Cultured
  • Fluorescent Dyes / chemistry*
  • Mice
  • Neural Stem Cells / chemistry*
  • Neural Stem Cells / physiology
  • Time-Lapse Imaging / methods*

Substances

  • Fluorescent Dyes