CXCL5 drives neutrophil recruitment in TH17-mediated GN

J Am Soc Nephrol. 2015 Jan;26(1):55-66. doi: 10.1681/ASN.2013101061. Epub 2014 Jun 5.

Abstract

Neutrophil trafficking to sites of inflammation is essential for the defense against bacterial and fungal infections, but also contributes to tissue damage in TH17-mediated autoimmunity. This process is regulated by chemokines, which often show an overlapping expression pattern and function in pathogen- and autoimmune-induced inflammatory reactions. Using a murine model of crescentic GN, we show that the pathogenic TH17/IL-17 immune response induces chemokine (C-X-C motif) ligand 5 (CXCL5) expression in kidney tubular cells, which recruits destructive neutrophils that contribute to renal tissue injury. By contrast, CXCL5 was dispensable for neutrophil recruitment and effective bacterial clearance in a murine model of acute bacterial pyelonephritis. In line with these findings, CXCL5 expression was highly upregulated in the kidneys of patients with ANCA-associated crescentic GN as opposed to patients with acute bacterial pyelonephritis. Our data therefore identify CXCL5 as a potential therapeutic target for the restriction of pathogenic neutrophil infiltration in TH17-mediated autoimmune diseases while leaving intact the neutrophil function in protective immunity against invading pathogens.

Keywords: CXCL5; GN; IL-17; chemokines; immunology; neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemokine CXCL1 / metabolism
  • Chemokine CXCL5 / metabolism*
  • Chemokines / metabolism
  • Disease Models, Animal
  • Epithelial Cells / cytology
  • Female
  • Glomerulonephritis / metabolism
  • Glomerulonephritis / microbiology
  • Glomerulonephritis / pathology*
  • Inflammation
  • Interleukin-17 / metabolism
  • Kidney / metabolism
  • Kidney Tubules / metabolism
  • Male
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Neutrophil Infiltration / immunology
  • Neutrophils / metabolism*
  • Th17 Cells / cytology*
  • Up-Regulation

Substances

  • Chemokine CXCL1
  • Chemokine CXCL5
  • Chemokines
  • Cxcl1 protein, mouse
  • Cxcl5 protein, mouse
  • Interleukin-17