Abstract
Adenocarcinoma of the colon in 10-20% is associated with microsatellite instability, which can occur both in sporadic cancers and in hereditary nonpolyposis colon cancer. Our analysis of 195 cases of adenocarcinoma of the colon showed that microsatellite instability (MSI-H) was found only in 1.5% of patients. Subsequent choice of patients with suspected hereditary Lynch syndrome led to the identification of additional 17 patients with microsatellite instability. They passed an analysis of genes of repair system of unpaired nucleotides of DNA. The study showed that immunohistochemical staining of MSH2, MSH6, MLH1, PMS2 could effectively conduct a preliminary screening of the Lynch syndrome but was unable to divide cases of sporadic and hereditary MSI-H colon cancer.
MeSH terms
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Adaptor Proteins, Signal Transducing / analysis*
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Adenocarcinoma / chemistry*
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Adenocarcinoma / diagnosis
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Adenocarcinoma / genetics
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Adenosine Triphosphatases / analysis*
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Adult
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Aged
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Biomarkers, Tumor / analysis*
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Colonic Neoplasms / chemistry*
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Colonic Neoplasms / diagnosis
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Colonic Neoplasms / genetics
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Colorectal Neoplasms, Hereditary Nonpolyposis / chemistry*
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Colorectal Neoplasms, Hereditary Nonpolyposis / diagnosis
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DNA Repair Enzymes / analysis*
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DNA-Binding Proteins / analysis*
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Female
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Humans
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Immunohistochemistry
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Male
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Microsatellite Instability*
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Middle Aged
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Mismatch Repair Endonuclease PMS2
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MutL Protein Homolog 1
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MutS Homolog 2 Protein / analysis*
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Nuclear Proteins / analysis*
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Predictive Value of Tests
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Sensitivity and Specificity
Substances
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Adaptor Proteins, Signal Transducing
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Biomarkers, Tumor
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DNA-Binding Proteins
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G-T mismatch-binding protein
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MLH1 protein, human
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Nuclear Proteins
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Adenosine Triphosphatases
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PMS2 protein, human
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MSH2 protein, human
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Mismatch Repair Endonuclease PMS2
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MutL Protein Homolog 1
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MutS Homolog 2 Protein
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DNA Repair Enzymes