Riboadenosine-substituted DNA probes for self-illuminating real-time monitoring of exonuclease III activity and exonuclease III-assisted target recycling

Chem Commun (Camb). 2014 Jul 28;50(59):8047-9. doi: 10.1039/c4cc03021d.

Abstract

We develop a riboadenosine-substituted DNA probe-based bioluminescence method for real-time monitoring of exonuclease III (Exo III) activity and Exo III-assisted target recycling. This method employs the target-dependent ATP as the bioluminescence reporter without the involvement of any sophisticated luciferase manipulations, and the glow-type reaction kinetics facilitates the performance of imaging assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / chemistry*
  • Adenosine / metabolism
  • DNA Probes / chemistry*
  • DNA Probes / metabolism
  • Enzyme Activation
  • Exodeoxyribonucleases / metabolism*
  • Kinetics
  • Luminescent Measurements*
  • Time Factors

Substances

  • DNA Probes
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III
  • Adenosine