Cloning and characterization of cytoplasmic dynein intermediate chain in Fenneropenaeus chinensis and its essential role in white spot syndrome virus infection

Jixing Feng; Xiaoqian Tang; Wenbin Zhan

doi:10.1016/j.fsi.2014.05.034

Cloning and characterization of cytoplasmic dynein intermediate chain in Fenneropenaeus chinensis and its essential role in white spot syndrome virus infection

Fish Shellfish Immunol. 2014 Aug;39(2):407-14. doi: 10.1016/j.fsi.2014.05.034. Epub 2014 Jun 9.

Authors

Jixing Feng¹, Xiaoqian Tang¹, Wenbin Zhan²

Affiliations

¹ Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
² Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China. Electronic address: [email protected].

PMID: 24925758
DOI: 10.1016/j.fsi.2014.05.034

Abstract

To investigate the role of cytoplasmic dynein in white spot syndrome virus (WSSV) infection, the full-length cDNA of cytoplasmic dynein intermediate chain (FcDYNCI) was cloned in Fenneropenaeus chinensis, which consists of 2582 bp and encodes a polypeptide of 660 amino acids. Sequence analysis and multiple sequence alignment displayed that FcDYNCI was a member of cytoplasmic dynein 1 family. The FcDYNCI mRNA was most highly expressed in hemocytes, which was significantly up-regulated post WSSV infection. At 12 h post infection (hpi), confocal microscopic observation showed that WSSV could be co-localized with cytoplasmic dynein in hemocytes. After silencing by specific FcDYNCI dsRNA, the FcDYNCI mRNA level and the protein amount of FcDYNCI in hemocytes both exhibited a significant reduction, and the expression levels of three WSSV genes ie1, wsv477 and vp28 all exhibited the greatest decreases at 24 hpi. These results suggested that cytoplasmic dynein was involved in WSSV infection.

Keywords: Cytoplasmic dynein; Fenneropenaeus chinensis; RNA interfere; White spot syndrome virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cloning, Molecular
DNA Primers / genetics
DNA, Complementary / genetics
Dyneins / genetics*
Dyneins / metabolism*
Gene Expression Regulation / immunology*
Gene Expression Regulation, Viral / genetics*
Hemocytes / metabolism
Microscopy, Confocal
Molecular Sequence Data
Penaeidae / genetics*
Penaeidae / immunology
Penaeidae / virology*
RNA Interference
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Virion / metabolism
White spot syndrome virus 1*

Substances

DNA Primers
DNA, Complementary
Dyneins