Highly selective turn-on fluorescent sensor for nanomolar detection of biologically important Zn2+ based on isonicotinohydrazide derivative: application in cellular imaging

Biosens Bioelectron. 2014 Nov 15:61:429-33. doi: 10.1016/j.bios.2014.05.053. Epub 2014 Jun 4.

Abstract

A new Zn(2+) selective chemosensor (3) was synthesized by condensation of commercially available substituted salicylaldehyde and isonicotinohydrazide, and characterized by single crystal X-ray crystallography. Receptor 3 with Zn(2+) exhibited a highly selective and pronounced enhancement in the fluorescence emission among different cations by forming a 2:1 complex. The receptor can detect Zn(2+) up to nanomolar level (6.75 nM) with good tolerance of other metal ions and can be used for in vitro cellular imaging.

Keywords: Cellular imaging; Fluorescent sensor; Nanomolar detection; Zn(2+) detection.

MeSH terms

  • Aldehydes / chemistry*
  • Cations, Divalent / analysis
  • Crystallography, X-Ray
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Isoniazid / analogs & derivatives*
  • Microscopy, Fluorescence / methods
  • Optical Imaging / methods*
  • Zinc / analysis*

Substances

  • Aldehydes
  • Cations, Divalent
  • Fluorescent Dyes
  • salicylaldehyde
  • Zinc
  • Isoniazid