Deciphering the cis-regulatory elements for XYR1 and CRE1 regulators in Trichoderma reesei

PLoS One. 2014 Jun 18;9(6):e99366. doi: 10.1371/journal.pone.0099366. eCollection 2014.

Abstract

In this work, we report the in silico identification of the cis-regulatory elements for XYR1 and CRE1 proteins in the filamentous fungus Trichoderma reesei, two regulators that play a central role in the expression of cellulase genes. Using four datasets of condition-dependent genes from RNA-seq and RT-qPCR experiments, we performed unsupervised motif discovery and found two short motifs resembling the proposed binding consensus for XYR1 and CRE1. Using these motifs, we analysed the presence and arrangement of putative cis-regulatory elements recognized by both regulators and found that shortly spaced sites were more associated with XYR1- and CRE1-dependent promoters than single, high-score sites. Furthermore, the approach used here allowed the identification of the previously reported XYR1-binding sites from cel7a and xyn1 promoters, and we also mapped the potential target sequence for this regulator at the cel6a promoter that has been suggested but not identified previously. Additionally, seven other promoters (for cel7b, cel61a, cel61b, cel3c, cel3d, xyn3 and swo genes) presented a putative XYR1-binding site, and strong sites for CRE1 were found at the xyr1 and cel7b promoters. Using the cis-regulatory architectures nearly defined for XYR1 and CRE1, we performed genome-wide identification of potential targets for direct regulation by both proteins and important differences on their functional regulons were elucidated. Finally, we performed binding site mapping on the promoters of differentially expressed genes found in T. reesei mutant strains lacking xyr1 or cre1 and found that indirect regulation plays a key role on their signalling pathways. Taken together, the data provided here sheds new light on the mechanisms for signal integration mediated by XYR1 and CRE1 at cellulase promoters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Consensus Sequence
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Promoter Regions, Genetic
  • Regulatory Elements, Transcriptional
  • Sequence Analysis, DNA
  • Trichoderma / genetics*

Substances

  • DNA, Fungal
  • Fungal Proteins

Grants and funding

This work was supported by The State of São Paulo Research Foundation (FAPESP, 2010/15683-8). RS-R holds a FAPESP post-doctoral Fellowship (FAPESP, 2013/04125-2). MEG benefits from a Young Talent Fellowship of the National Counsel of Technological and Scientific Development (CNPq, Ref. Number 370630/2013-0). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.