Abstract
CL26 murine colon carcinoma cells express urokinase-type plasminogen activator (u-PA) mRNA and activity after transfection with the activated c-Ha-ras-I (EJ-ras) oncogene cloned from the EJ bladder carcinoma. PA activity and mRNA in control cells transfected with the non-mutated c-Ha-ras-I (CO-ras) gene remained negative. Ras mRNA was detected in EJ-ras- and CO-ras-transfected cells, but not in untransfected or pSV2-neo-transfected cells. These results indicate that u-PA biosynthesis can be modulated by EJ-Ha-ras-dependent pathways of signal transduction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Cell Line
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Cells, Cultured
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Colonic Neoplasms / enzymology*
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Colonic Neoplasms / genetics
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Colonic Neoplasms / pathology
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Enzyme Induction
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Enzyme Precursors / biosynthesis
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Enzyme Precursors / genetics*
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Genes, ras*
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Mice
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Plasmids
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Plasminogen Activators / biosynthesis
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Plasminogen Activators / genetics*
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RNA, Messenger / genetics
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Transcription, Genetic*
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Transfection
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Urokinase-Type Plasminogen Activator / biosynthesis
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Urokinase-Type Plasminogen Activator / genetics*
Substances
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Enzyme Precursors
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RNA, Messenger
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Plasminogen Activators
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Urokinase-Type Plasminogen Activator