Abstract
Parathyroid hormone-related protein (PTHrP) has been identified to play a crucial role in osteolysis formation and stromal cell (GCTSC) proliferation in giant cell tumor (GCT). MiR-126-5p is an intronic miRNA identified as tumor suppressor in many tumors, but its role in GCT is poorly understood. We found that miR-126-5p was decreased in GCT and could directly regulate PTHrP expression. Furthermore, miR-126-5p could control osteoclast (OC) differentiation, GCTSC proliferation and osteolysis formation in GCT through negative regulation of PTHrP. Thus, these results suggest that miR-126-5p could directly target PTHrP and have a tumor suppressor function in GCT.
Keywords:
Giant cell tumor; Osteoclast differentiation; Osteolysis; PTHrP; miR-126-5p.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Bone Resorption / pathology
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Cell Cycle Checkpoints
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Cell Line, Tumor
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Cell Proliferation
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Down-Regulation / genetics
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Endonucleases / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Genetic Engineering
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Giant Cell Tumors / complications*
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Giant Cell Tumors / genetics
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Giant Cell Tumors / pathology*
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Giant Cells / metabolism
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Giant Cells / pathology
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HEK293 Cells
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Humans
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Mice, Inbred C57BL
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Molecular Sequence Data
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Osteolysis / etiology*
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Osteolysis / genetics
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Osteolysis / pathology*
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Parathyroid Hormone-Related Protein / antagonists & inhibitors*
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Parathyroid Hormone-Related Protein / metabolism
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Stromal Cells / metabolism
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Stromal Cells / pathology
Substances
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MIRN126 microRNA, human
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MicroRNAs
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Parathyroid Hormone-Related Protein
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Endonucleases