Two-photon imaging of neural activity in awake mobile mice

Cold Spring Harb Protoc. 2014 Jul 1;2014(7):726-36. doi: 10.1101/pdb.top081810.

Abstract

A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain's microcircuitry is connected to animal behavior.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diagnostic Imaging / methods*
  • Exercise Test
  • Mice
  • Microscopy, Fluorescence, Multiphoton*
  • Neurons / physiology*
  • Photons*
  • Wakefulness