Abstract
A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocatalysis
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Enzymes, Immobilized / chemistry*
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Enzymes, Immobilized / metabolism*
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Glass
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Lipase / chemistry
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Lipase / metabolism
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Oxygenases / chemistry
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Oxygenases / metabolism
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Polymers / chemistry
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Porosity
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Sterol Esterase / chemistry
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Sterol Esterase / metabolism
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Transaminases / chemistry
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Transaminases / metabolism
Substances
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Enzymes, Immobilized
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Fungal Proteins
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Polymers
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Oxygenases
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2,5-diketocamphane 1,2-monooxygenase
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Transaminases
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Sterol Esterase
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Lipase
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lipase B, Candida antarctica