Genetic diversity and molecular epidemiology of human rhinoviruses in South Africa

Influenza Other Respir Viruses. 2014 Sep;8(5):567-73. doi: 10.1111/irv.12264. Epub 2014 Jul 3.

Abstract

Background: Rhinoviruses (RV) are a well-established cause of respiratory illness. RV-C has been associated with more severe illness. We aimed to characterize and compare the clinical presentations and disease severity of different RV type circulating in South Africa.

Method: We performed two analyses of RV-positive specimens identified through surveillance in South Africa across all age groups. First, RV-positive specimens identified through severe acute respiratory illness (SARI) surveillance in four provinces was randomly selected from 2009 to 2010 for molecular characterization. Second, RV-positive specimens identified through SARI, influenza-like illness (ILI) and control surveillance at hospitals and outpatient clinics in during 2012-2013 were used to determine the association of RV type with severe disease. Selected specimens were sequenced, and phylogenetic analysis was performed.

Results: Among the 599 sequenced specimens from 2009 to 2010 and 2012 to 2013, RV-A (285, 48%) and RV-C (247, 41%) were more commonly identified than RV-B (67, 11%), with no seasonality and a high genetic diversity. A higher prevalence of RV infection was identified in cases with SARI [515/962 (26%); aRRR = 1.6; 95% CI 1.21; 2.2] and ILI [356/962 (28%); aRRR = 1.9; 95% CI 1.37; 2.6] compared with asymptomatic controls (91/962, 22%). There was no difference in disease severity between the different type when comparing SARI, ILI and controls.

Conclusion: All three type of RV were identified in South Africa, although RV-A and RV-C were more common than RV-B. RV was associated with symptomatic respiratory illness; however, there was no association between RV type and disease severity.

Keywords: Disease association; South Africa; genetic diversity; rhinovirus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Female
  • Genetic Variation*
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Phylogeny
  • Picornaviridae Infections / epidemiology
  • Picornaviridae Infections / virology*
  • Rhinovirus / classification
  • Rhinovirus / genetics*
  • Rhinovirus / isolation & purification*
  • South Africa / epidemiology
  • Young Adult