Gestational age-specific reference ranges of hepcidin in cord blood

Neonatology. 2014;106(2):133-9. doi: 10.1159/000360072. Epub 2014 Jun 27.

Abstract

Background: Iron deficiency (ID) contributes to anaemia of prematurity, and hence the reliable assessment of iron nutrition status appears to be mandatory.

Objective: To establish gestational age (GA)-specific reference ranges for hepcidin concentrations in cord blood [Hep(CB)] of preterm and term infants and to identify pre- and perinatal confounding factors.

Methods: This is a prospective observational study including 221 infants (GA at birth: 24-42 weeks). Hep(CB) along with complete blood counts, ferritin and parameters of inflammation and clinical data were recorded. Data are presented as medians (IQR).

Results: The Hep(CB) of very preterm infants (GA <30 weeks, n = 40) was 26.9 ng/ml (13.5-63.1), for moderately preterm infants (GA 30-36 weeks, n = 81) it was 45.9 ng/ml (24.7-74.5) and for term infants (GA ≥37 weeks, n = 100) it was 103.9 ng/ml (61.4-149.2). The Hep(CB) of infants with ID was lower [36.9 ng/ml (18.0-58.3)] than that of iron-replete infants [86.6 ng/ml (51.9-143.8)]. The Hep(CB) of infants delivered by elective caesarean section was lower [38.3 ng/ml (15.5-73.7)] than that of infants after spontaneous vaginal delivery or secondary caesarean section [80.3 ng/ml (48.5-137.6)]. Infants with a standard deviation score for birth weight (SDSBW) <-2 had a lower Hep(CB) [23.1 ng/ml (11.7-61.5)] compared to infants with SDSBW ≥-2 [71.1 ng/ml (34.0-121.7)]. The highest Hep(CB) (437.6 ng/ml) was recorded in an infant with Enterococcus faecalis sepsis. Multiple logistic regression analysis confirmed ferritin, GA and mode of delivery as important factors associated with Hep(CB).

Conclusion: This is the first report on GA-specific reference ranges for Hep(CB) in preterm infants. Whereas iron stores, GA and mode of delivery were associated with Hep(CB), the association with inflammation and intra-uterine growth retardation was less clear.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Anemia, Iron-Deficiency / blood*
  • Anemia, Iron-Deficiency / diagnosis
  • Biomarkers / blood
  • Birth Weight
  • Case-Control Studies
  • Delivery, Obstetric
  • Enterococcus faecalis / isolation & purification
  • Female
  • Ferritins / blood
  • Fetal Blood / chemistry*
  • Fetal Growth Retardation / blood
  • Fetal Growth Retardation / diagnosis
  • Gestational Age
  • Gram-Positive Bacterial Infections / blood
  • Gram-Positive Bacterial Infections / diagnosis
  • Gram-Positive Bacterial Infections / microbiology
  • Hemoglobins / analysis
  • Hepcidins / blood*
  • Humans
  • Infant, Low Birth Weight / blood
  • Infant, Newborn
  • Infant, Premature / blood
  • Inflammation Mediators / blood
  • Logistic Models
  • Male
  • Multivariate Analysis
  • Odds Ratio
  • Predictive Value of Tests
  • Prospective Studies
  • Reference Values
  • Sepsis / blood
  • Sepsis / diagnosis
  • Sepsis / microbiology

Substances

  • Biomarkers
  • Hemoglobins
  • Hepcidins
  • Inflammation Mediators
  • Ferritins