o-quinone/quinone methide isomerase: a novel enzyme preventing the destruction of self-matter by phenoloxidase-generated quinones during immune response in insects

FEBS Lett. 1989 Jun 5;249(2):155-8. doi: 10.1016/0014-5793(89)80614-3.

Abstract

Melanization and encapsulation of invading foreign organisms observed during the immune response in insects is known to be due to the action of activated phenoloxidase. Phenoloxidase-generated quinones are deposited either directly or after self-polymerization on foreign objects accounting for the observed reactions. Since the reactions of quinones are nonenzymatic, they do not discriminate self from nonself and hence will also destroy self-matter. In this report we present evidence for the presence of a novel quinone/quinone methide isomerase in the hemolymph of Sarcophaga bullata which destroys long-lived quinones and hence acts to protect the self-matter. Quinone methides, formed by the action of this enzyme on physiologically important quinones, being unstable undergo rapid hydration to form nontoxic metabolites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Catechol Oxidase / metabolism*
  • Chemical Phenomena
  • Chemistry
  • Chromatography, High Pressure Liquid
  • Dopamine / analogs & derivatives
  • Dopamine / metabolism
  • Hemolymph / enzymology*
  • Insecta / immunology*
  • Insecta / metabolism
  • Intramolecular Oxidoreductases*
  • Isomerases / metabolism*
  • Monophenol Monooxygenase / metabolism*
  • Quinones / metabolism*
  • Spectrophotometry, Ultraviolet

Substances

  • Quinones
  • N-acetyldopamine
  • Catechol Oxidase
  • Monophenol Monooxygenase
  • Isomerases
  • Intramolecular Oxidoreductases
  • quinone methide isomerase
  • Dopamine