A DNA minor groove binder shows high effectiveness as a quencher for FRET probes

Bioorg Med Chem Lett. 2014 Aug 15;24(16):3956-60. doi: 10.1016/j.bmcl.2014.06.036. Epub 2014 Jun 19.

Abstract

A non-fluorescent quencher based on thiazole orange was incorporated into oligonucleotides. Fluorimetry and fluorogenic real-time polymerase chain reaction experiments demonstrated that the quencher is effective for fluorescein amidite dyes. The thiazole orange quencher also increased the melting temperature of DNA duplexes, which may facilitate the design of shorter and more discriminatory probes. The effectiveness of the quencher in TaqMan probes was also demonstrated.

Keywords: Fluorescein amidite; Non-fluorescent quencher; Thiazole orange.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles / chemistry*
  • DNA / chemistry*
  • DNA Probes / chemistry*
  • DNA Probes / genetics
  • Fluorescence Resonance Energy Transfer*
  • Fluorescent Dyes
  • Quinolines / chemistry*
  • Real-Time Polymerase Chain Reaction

Substances

  • Benzothiazoles
  • DNA Probes
  • Fluorescent Dyes
  • Quinolines
  • thiazole orange
  • DNA