Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

Satoru Watanabe; Jun Sato; Sousuke Imamura; Mio Ohnuma; Yusaku Ohoba; Taku Chibazakura; Kan Tanaka; Hirofumi Yoshikawa

doi:10.1080/09168451.2014.877823

Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

Biosci Biotechnol Biochem. 2014;78(1):175-7. doi: 10.1080/09168451.2014.877823. Epub 2014 Apr 16.

Authors

Satoru Watanabe¹, Jun Sato, Sousuke Imamura, Mio Ohnuma, Yusaku Ohoba, Taku Chibazakura, Kan Tanaka, Hirofumi Yoshikawa

Affiliation

¹ a Department of Bioscience , Tokyo University of Agriculture , Tokyo , Japan.

PMID: 25036501
DOI: 10.1080/09168451.2014.877823

Abstract

The unicellular red alga Cyanidioschyzon merolae is used as a model organism to investigate the basic architecture of photosynthetic eukaryotes. We established a stable expression system for the green fluorescent protein fused with the phycocyanin-associated rod linker (APCC) protein in C. merolae, which was clearly localized on the plastid. This system should be useful in the genetic engineering of C. merolae.

Keywords: APCC; Cyanidioschyzon merolae; green fluorescent protein (GFP); transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression
Genes, Reporter / genetics*
Genomics
Green Fluorescent Proteins / genetics*
Rhodophyta / cytology
Rhodophyta / genetics*
Transformation, Genetic

Substances

Green Fluorescent Proteins