Abstract
In this study, we use antisera to synthetic beta-amyloid protein precursor (beta APP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble approximately 125- and approximately 105-kDa derivatives of the beta APP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble approximately 125-kDa beta APP derivative contains the Kunitz protease inhibitor domain, whereas the approximately 105-kDa form does not, and we confirm that these two proteins are soluble beta APP derivatives by purifying each from human CSF and directly sequencing its amino terminus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alzheimer Disease / cerebrospinal fluid
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Alzheimer Disease / metabolism*
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Amino Acid Sequence
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Amyloid / chemical synthesis
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Amyloid / genetics
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Amyloid / isolation & purification*
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Amyloid beta-Protein Precursor
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Brain / metabolism*
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Cerebral Cortex / metabolism
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Humans
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Immune Sera
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Immunoblotting
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Molecular Sequence Data
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Molecular Weight
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Protein Precursors / chemical synthesis
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Protein Precursors / genetics
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Protein Precursors / isolation & purification*
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Transfection
Substances
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Amyloid
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Amyloid beta-Protein Precursor
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Immune Sera
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Protein Precursors