Bioinformatic exploration of RIO protein kinases of parasitic and free-living nematodes

Int J Parasitol. 2014 Oct 1;44(11):827-36. doi: 10.1016/j.ijpara.2014.06.005. Epub 2014 Jul 17.

Abstract

Despite right open reading frame kinases (RIOKs) being essential for life, their functions, substrates and cellular pathways remain enigmatic. In the present study, gene structures were characterised for 26 RIOKs from draft genomes of parasitic and free-living nematodes. RNA-seq transcription profiles of riok genes were investigated for selected parasitic nematodes and showed that these kinases are transcribed in developmental stages that infect their mammalian host. Three-dimensional structural models of Caenorhabditis elegans RIOKs were predicted, and elucidated functional domains and conserved regions in nematode homologs. These findings provide prospects for functional studies of riok genes in C. elegans, and an opportunity for the design and validation of nematode-specific inhibitors of these enzymes in socioeconomic parasitic worms.

Keywords: Function; Parasitic nematode; RIO kinase; Structure.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Computational Biology*
  • Gene Expression Regulation, Enzymologic / physiology
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • Models, Molecular
  • Nematoda / enzymology*
  • Nematoda / genetics
  • Nematoda / metabolism
  • Phosphotransferases / genetics
  • Phosphotransferases / metabolism*
  • Phylogeny
  • Protein Conformation
  • Transcriptome

Substances

  • Helminth Proteins
  • Phosphotransferases