The premature seeds of D. huoshanense collected in September or October can develop normally in MS or Kundson media with BAI and NAA 0.5 mg/L and form complete plantlets. The premature seeds cultured in dark for 22 days and then in low light intensity give the highest germination percent age(91%). The suitable temperature for the germination of D. huoshanense premature seeds is 25-27 degrees C.