Abstract
In 210 strains of Enterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20 micrograms) + clavulanate (10 micrograms) and cefotaxime (30 micrograms) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrum beta-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
MeSH terms
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Amoxicillin / pharmacology*
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Amoxicillin-Potassium Clavulanate Combination
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Cefotaxime / pharmacology*
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Citrobacter / drug effects
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Citrobacter / enzymology
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Citrobacter / isolation & purification
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Clavulanic Acids / pharmacology*
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Drug Resistance, Microbial
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Drug Synergism
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Drug Therapy, Combination / pharmacology
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Enterobacter / drug effects
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Enterobacter / enzymology
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Enterobacter / isolation & purification
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / isolation & purification
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Escherichia coli / isolation & purification
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France
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Humans
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Microbial Sensitivity Tests
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Penicillin Resistance
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Serratia marcescens / drug effects
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Serratia marcescens / enzymology
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Serratia marcescens / isolation & purification
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beta-Lactamases / metabolism*
Substances
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Clavulanic Acids
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Amoxicillin-Potassium Clavulanate Combination
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Amoxicillin
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beta-Lactamases
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Cefotaxime