Relations between vitamin D and fatty acid binding properties of vitamin D-binding protein

Biochem Biophys Res Commun. 1989 Aug 30;163(1):14-7. doi: 10.1016/0006-291x(89)92091-3.

Abstract

The interaction of fatty acids with bovine vitamin D-binding protein (DBP) was studied using a partition equilibrium method. This protein has one high affinity site for binding of fatty acids with an association constant Ka = 7 x 10(5) M-1 for palmitic acid and Ka = 6 x 10(5) M-1 for arachidonic acid. Competition experiments showed that palmitic acid hardly competes with 25-hydroxycholecalciferol for binding to DBP. However, arachidonic acid showed comparatively a stronger competition for binding to this protein. The great difference in competition of palmitic and arachidonic acids with 25-hydroxycholecalciferol may be related to changes in DBP conformation promoted by the binding of different ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acid
  • Arachidonic Acids / metabolism
  • Binding, Competitive
  • Calcifediol / metabolism
  • Cattle
  • Fatty Acids / metabolism*
  • In Vitro Techniques
  • Palmitic Acid
  • Palmitic Acids / metabolism
  • Protein Binding
  • Vitamin D / metabolism*
  • Vitamin D-Binding Protein / metabolism*

Substances

  • Arachidonic Acids
  • Fatty Acids
  • Palmitic Acids
  • Vitamin D-Binding Protein
  • Vitamin D
  • Arachidonic Acid
  • Palmitic Acid
  • Calcifediol