Dopaminergic neuron destruction reduces hippocampal serotonin 1A receptor uptake of trans-[(18)F]Mefway

Appl Radiat Isot. 2014 Dec:94:30-34. doi: 10.1016/j.apradiso.2014.06.016. Epub 2014 Jul 8.

Abstract

The purpose of the present study is to investigate the relationship between dopaminergic neuron destruction and 5-HT system changes in a hemiparkinsonian rat model. We performed PET imaging studies with trans-[(18)F]Mefway in a hemiparkinsonian model of unilateral 6-hydroxydopamine (6-OHDA) rats. Region-of-interests (ROIs) were drawn in the hippocampus (HP) and cerebellum (CB). HP uptake, the ratios of specific binding to non-specific binding in the HP, and non-displaceable binding potential (BPND) in the HP were compared between 6-OHDA and control rats. As a result, unilateral 6-OHDA-lesioned rats exhibited significant bilateral reduction of HP uptake and trans-[(18)F]Mefway BPND compared to the intact control group. Therefore, the results demonstrate that destruction of the dopaminergic system causes the reduction of the serotonergic system.

Keywords: 5-HT(1A) receptors; 6-hydroxydopamine; Hemiparkinsonian model; MicroPET; trans-[(18)F]Mefway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dopaminergic Neurons / diagnostic imaging
  • Dopaminergic Neurons / metabolism*
  • Fluorine Radioisotopes / pharmacokinetics
  • Hippocampus / diagnostic imaging
  • Hippocampus / metabolism*
  • Male
  • Metabolic Clearance Rate
  • Parkinsonian Disorders / diagnostic imaging
  • Parkinsonian Disorders / metabolism*
  • Piperazines / pharmacokinetics*
  • Pyridines / pharmacokinetics*
  • Radionuclide Imaging
  • Radiopharmaceuticals / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Serotonin, 5-HT1A / metabolism*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tissue Distribution

Substances

  • Fluorine Radioisotopes
  • Piperazines
  • Pyridines
  • Radiopharmaceuticals
  • mefway
  • Receptor, Serotonin, 5-HT1A