Systemic gene silencing in plants triggered by fluorescent nanoparticle-delivered double-stranded RNA

Li Jiang; Lian Ding; Bicheng He; Jie Shen; Zejun Xu; Meizhen Yin; Xiaolan Zhang

doi:10.1039/c4nr03481c

Systemic gene silencing in plants triggered by fluorescent nanoparticle-delivered double-stranded RNA

Nanoscale. 2014 Sep 7;6(17):9965-9. doi: 10.1039/c4nr03481c.

Authors

Li Jiang¹, Lian Ding, Bicheng He, Jie Shen, Zejun Xu, Meizhen Yin, Xiaolan Zhang

Affiliation

¹ College of Agronomy and Biotechnology, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China. [email protected] [email protected].

PMID: 25068243
DOI: 10.1039/c4nr03481c

Abstract

A cationic fluorescence nanoparticle efficiently enters plants with high transfection efficacy. Applying a mixture of G2/dsRNA to the model plant, Arabidopsis root, leads to significant reduction in the expression of important developmental genes and results in apparent phenotypes. This study reports a non-viral gene nanocarrier which triggers gene silencing in plants and leads to systemic phenotypes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / cytology
Arabidopsis / genetics*
Fluorescent Dyes / chemistry*
Materials Testing
Nanocapsules / administration & dosage
Nanocapsules / chemistry*
Nanocapsules / ultrastructure
Particle Size
Plants, Genetically Modified / genetics
RNA Interference*
RNA, Double-Stranded / administration & dosage
RNA, Double-Stranded / genetics*
Surface Properties
Transfection / methods*

Substances

Fluorescent Dyes
Nanocapsules
RNA, Double-Stranded