Detection of rare antigen-presenting cells through T cell-intrinsic meandering motility, mediated by Myo1g

Cell. 2014 Jul 31;158(3):492-505. doi: 10.1016/j.cell.2014.05.044.

Abstract

To mount an immune response, T lymphocytes must successfully search for foreign material bound to the surface of antigen-presenting cells. How T cells optimize their chances of encountering and responding to these antigens is unknown. T cell motility in tissues resembles a random or Levy walk and is regulated in part by external factors including chemokines and lymph-node topology, but motility parameters such as speed and propensity to turn may also be cell intrinsic. Here we found that the unconventional myosin 1g (Myo1g) motor generates membrane tension, enforces cell-intrinsic meandering search, and enhances T-DC interactions during lymph-node surveillance. Increased turning and meandering motility, as opposed to ballistic motility, is enhanced by Myo1g. Myo1g acts as a "turning motor" and generates a form of cellular "flânerie." Modeling and antigen challenges show that these intrinsically programmed elements of motility search are critical for the detection of rare cognate antigen-presenting cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • Cell Membrane / metabolism
  • Cell Movement
  • Immunologic Surveillance*
  • Lymph Nodes / immunology
  • Mice
  • Minor Histocompatibility Antigens
  • Myosins / genetics
  • Myosins / metabolism*
  • Receptors, Antigen, T-Cell / metabolism
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • Minor Histocompatibility Antigens
  • Myo1g protein, mouse
  • Receptors, Antigen, T-Cell
  • Myosins