Cyclic phosphatidic acid treatment suppress cuprizone-induced demyelination and motor dysfunction in mice

Eur J Pharmacol. 2014 Oct 15:741:17-24. doi: 10.1016/j.ejphar.2014.07.040. Epub 2014 Jul 30.

Abstract

Multiple sclerosis is a chronic demyelinating disease of the central nervous system leading to progressive cognitive and motor dysfunction, which is characterized by neuroinflammation, demyelination, astrogliosis, loss of oligodendrocytes, and axonal pathologies. Cyclic phosphatidic acid (cPA) is a naturally occurring phospholipid mediator with a unique cyclic phosphate ring structure at the sn-2 and sn-3 positions of the glycerol backbone. cPA elicits a neurotrophin-like action and protects hippocampal neurons from ischemia-induced delayed neuronal death. In this study, we investigated the effects of cPA on cuprizone-induced demyelination, which is a model of multiple sclerosis. Mice were fed a diet containing 0.2% cuprizone for 5 weeks, which induces severe demyelination, astrocyte and microglial activation, and motor dysfunction. Simultaneous administration of cPA effectively attenuated cuprizone-induced demyelination, glial activation, and motor dysfunction. These data indicate that cPA may be a useful treatment to reduce the extent of demyelination and the severity of motor dysfunction in multiple sclerosis. cPA is a potential lead compound in the development of drugs for the treatment of this devastating disease.

Keywords: Cuprizone (CPZ) (PubChem CID: 9723); Cyclic phosphatidic acid (cPA); Demyelination; Multiple sclerosis (MS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cuprizone / toxicity*
  • Demyelinating Diseases / chemically induced
  • Demyelinating Diseases / pathology
  • Demyelinating Diseases / prevention & control*
  • Disease Models, Animal*
  • Heterocyclic Compounds, 1-Ring / pharmacology
  • Heterocyclic Compounds, 1-Ring / therapeutic use*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Motor Activity / drug effects
  • Motor Skills Disorders / chemically induced
  • Motor Skills Disorders / pathology
  • Motor Skills Disorders / prevention & control*
  • Phosphatidic Acids / pharmacology
  • Phosphatidic Acids / therapeutic use*
  • Treatment Outcome

Substances

  • Heterocyclic Compounds, 1-Ring
  • Phosphatidic Acids
  • cyclic phosphatidic acid 16:0
  • Cuprizone