Purification and culture of dorsal root ganglion neurons

J Bradley Zuchero

doi:10.1101/pdb.top073965

Purification and culture of dorsal root ganglion neurons

Cold Spring Harb Protoc. 2014 Aug 1;2014(8):813-4. doi: 10.1101/pdb.top073965.

Author

J Bradley Zuchero¹

Affiliation

¹ Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.

Abstract

Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.

Publication types

Introductory Journal Article

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Separation / methods*
Culture Media, Serum-Free / chemistry
Ganglia, Spinal / cytology*
Neurons / physiology*
Rodentia

Substances

Culture Media, Serum-Free

Grants and funding

R01 EY010257/EY/NEI NIH HHS/United States