Serum neutralization assay can efficiently replace plaque reduction neutralization test for detection and quantitation of West Nile virus antibodies in human and animal serum samples

Annapia Di Gennaro; Alessio Lorusso; Claudia Casaccia; Annamaria Conte; Federica Monaco; Giovanni Savini

doi:10.1128/CVI.00426-14

Serum neutralization assay can efficiently replace plaque reduction neutralization test for detection and quantitation of West Nile virus antibodies in human and animal serum samples

Clin Vaccine Immunol. 2014 Oct;21(10):1460-2. doi: 10.1128/CVI.00426-14. Epub 2014 Aug 6.

Authors

Annapia Di Gennaro¹, Alessio Lorusso², Claudia Casaccia¹, Annamaria Conte¹, Federica Monaco¹, Giovanni Savini¹

Affiliations

¹ OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
² OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy [email protected].

Abstract

A serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / blood*
Antibodies, Viral / blood*
Horses
Humans
Neutralization Tests / methods*
Viral Plaque Assay / methods
West Nile virus / immunology*

Substances

Antibodies, Neutralizing
Antibodies, Viral