Cynoglossus puncticeps (Soleoidei Cynoglossidae) is characterized by both eyes on the left side of the body. Here we report the mitogenome of this tonguesole for the first time, which is 17,142 bp in length, and the gene order has been reorganized. The tRNA-Gln gene translocated from the light strand (L-strand) to the heavy strand (H-strand), accompanied by tRNA-Ile gene shuffling. In addition, the putative control region translocated downstream to the position between the ND1 and the tRNA-Gln genes, leaving a 25-bp trace fragment in the original location. In addition, two tandem arrays were found: one was a 17-bp motif with 40.2 copies, and the other was 73-bp with 3.0 copies.
Keywords: Mitogenome; mottled tonguesole; reorganization.