Murine xenograft model demonstrates significant radio-sensitising effect of liposomal doxorubicin in a combination therapy for Feline Injection Site Sarcoma

H Petznek; M Kleiter; A Tichy; A Fuchs-Baumgartinger; C Hohenadl

doi:10.1016/j.rvsc.2014.07.008

Murine xenograft model demonstrates significant radio-sensitising effect of liposomal doxorubicin in a combination therapy for Feline Injection Site Sarcoma

Res Vet Sci. 2014 Oct;97(2):386-90. doi: 10.1016/j.rvsc.2014.07.008. Epub 2014 Jul 21.

Authors

H Petznek¹, M Kleiter², A Tichy³, A Fuchs-Baumgartinger⁴, C Hohenadl⁵

Affiliations

¹ Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: [email protected].
² Radiooncology and Nuclear Medicine Platform, Department for Companion Animals and Horses, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
³ Bioinformatics and Biostatistics Platform, Department for Biomedical Sciences, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
⁴ Institute of Pathology and Forensic Veterinary Medicine, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
⁵ Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.

PMID: 25104320
DOI: 10.1016/j.rvsc.2014.07.008

Abstract

Therapy of cats suffering from feline injection site sarcomas (FISS) is still a challenging problem, as the recurrence rate after surgery is up to 70%. Four FISS-derived primary tumour cell lines and corresponding xenograft tumour mouse models were established to evaluate the efficacy of a concomitant chemo-/radiation therapy with doxorubicin. In vitro, strongly depending upon the timing of administration, pre-treatment with 0.25 µmol doxorubicin resulted in a significant enhancement of radiation-induced (3.5 Gy) tumour cell death. This result was confirmed in vivo, where only the combined chemo-/radiation therapy resulted in a significant reduction in tumour growth compared to the respective mono-therapies with either doxorubicin or radiation. These results support the use of the concomitant chemo-/radiation therapy for adjuvant treatment of FISS, particularly in advanced or recurrent disease where surgery alone is no longer feasible.

Keywords: Doxorubicin; FISS; Primary feline sarcoma cell culture; Radiation; Xenograft tumour mouse model.

MeSH terms

Animals
Antibiotics, Antineoplastic / therapeutic use*
Cat Diseases / therapy*
Cats
Cell Line, Tumor
Combined Modality Therapy
Disease Models, Animal
Dose-Response Relationship, Drug
Doxorubicin / analogs & derivatives*
Doxorubicin / therapeutic use
Female
In Vitro Techniques
Male
Mice
Mice, Nude
Neoplasms, Connective Tissue / therapy
Neoplasms, Connective Tissue / veterinary*
Polyethylene Glycols / therapeutic use
Radiotherapy / methods
Radiotherapy / veterinary*
Sarcoma / therapy
Sarcoma / veterinary*
Time Factors
Transplantation, Heterologous / methods
Transplantation, Heterologous / veterinary
Treatment Outcome
Xenograft Model Antitumor Assays / methods
Xenograft Model Antitumor Assays / veterinary*

Substances

Antibiotics, Antineoplastic
liposomal doxorubicin
Polyethylene Glycols
Doxorubicin