Combinatorial gene therapy accelerates bone regeneration: non-viral dual delivery of VEGF and BMP2 in a collagen-nanohydroxyapatite scaffold

Caroline M Curtin; Erica G Tierney; Kevin McSorley; Sally-Ann Cryan; Garry P Duffy; Fergal J O'Brien

doi:10.1002/adhm.201400397

Combinatorial gene therapy accelerates bone regeneration: non-viral dual delivery of VEGF and BMP2 in a collagen-nanohydroxyapatite scaffold

Adv Healthc Mater. 2015 Jan 28;4(2):223-7. doi: 10.1002/adhm.201400397. Epub 2014 Aug 13.

Authors

Caroline M Curtin¹, Erica G Tierney, Kevin McSorley, Sally-Ann Cryan, Garry P Duffy, Fergal J O'Brien

Affiliation

¹ Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland; Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland; Advanced Materials and BioEngineering Research (AMBER) Centre Royal College of Surgeons in Ireland & Trinity College Dublin, Dublin, Ireland.

PMID: 25125073
DOI: 10.1002/adhm.201400397

Abstract

Vascularization and bone repair are accelerated by a series of gene-activated scaffolds delivering both an angiogenic and an osteogenic gene. Stem cell-mediated osteogenesis in vitro, in addition to increased vascularization and bone repair by host cells in vivo, is enhanced using all systems while the use of the nanohydroxyapatite vector to deliver both genes markedly enhances bone healing.

Keywords: bone regeneration; collagen scaffolds; gene-activated scaffolds; mesenchymal stem cells; non-viral gene therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Morphogenetic Protein 2 / administration & dosage
Bone Morphogenetic Protein 2 / pharmacology*
Bone Regeneration / drug effects*
Collagen / pharmacology*
Drug Delivery Systems*
Durapatite / pharmacology*
Gene Transfer Techniques
Genetic Therapy*
Human Umbilical Vein Endothelial Cells / drug effects
Humans
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects
Mice
Neovascularization, Physiologic / drug effects
Polyethyleneimine / pharmacology
Rats
Tissue Scaffolds / chemistry*
Vascular Endothelial Growth Factor A / administration & dosage
Vascular Endothelial Growth Factor A / pharmacology*
Wound Healing / drug effects

Substances

Bone Morphogenetic Protein 2
Vascular Endothelial Growth Factor A
nano-hydroxyapatite-collagen
Polyethyleneimine
Collagen
Durapatite