The GABAergic parafacial zone is a medullary slow wave sleep-promoting center

Nat Neurosci. 2014 Sep;17(9):1217-24. doi: 10.1038/nn.3789. Epub 2014 Aug 17.

Abstract

Work in animals and humans has suggested the existence of a slow wave sleep (SWS)-promoting/electroencephalogram (EEG)-synchronizing center in the mammalian lower brainstem. Although sleep-active GABAergic neurons in the medullary parafacial zone (PZ) are needed for normal SWS, it remains unclear whether these neurons can initiate and maintain SWS or EEG slow-wave activity (SWA) in behaving mice. We used genetically targeted activation and optogenetically based mapping to examine the downstream circuitry engaged by SWS-promoting PZ neurons, and we found that this circuit uniquely and potently initiated SWS and EEG SWA, regardless of the time of day. PZ neurons monosynaptically innervated and released synaptic GABA onto parabrachial neurons, which in turn projected to and released synaptic glutamate onto cortically projecting neurons of the magnocellular basal forebrain; thus, there is a circuit substrate through which GABAergic PZ neurons can potently trigger SWS and modulate the cortical EEG.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electroencephalography
  • GABAergic Neurons / physiology*
  • Integrases / genetics
  • Male
  • Medulla Oblongata / cytology
  • Medulla Oblongata / physiology*
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Parabrachial Nucleus / cytology
  • Parabrachial Nucleus / physiology
  • Prosencephalon / cytology
  • Prosencephalon / physiology
  • Respiratory Center / cytology
  • Respiratory Center / physiology*
  • Sleep / physiology*
  • Sleep Deprivation / physiopathology
  • Vagus Nerve / cytology
  • Vagus Nerve / physiology
  • gamma-Aminobutyric Acid / physiology

Substances

  • gamma-Aminobutyric Acid
  • Cre recombinase
  • Integrases