Labeled protein recognition at a membrane bilayer interface by embedded synthetic receptors

Langmuir. 2014 Sep 2;30(34):10161-6. doi: 10.1021/la502629d. Epub 2014 Aug 19.

Abstract

Self-folding deep cavitands embedded in a supported lipid bilayer are capable of recognizing suitably labeled proteins at the bilayer interface. The addition of a choline derived binding "handle" to a number of different proteins allows their selective noncovalent recognition, with association constants on the order of 10(5) M(-1). The proteins are displayed at the water:bilayer interface, and a single binding handle allows recognition of the large, charged protein by a small molecule synthetic receptor via complementary shape and charge interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Lipid Bilayers*
  • Proteins / chemistry*
  • Spectrometry, Mass, Electrospray Ionization
  • Surface Plasmon Resonance

Substances

  • Lipid Bilayers
  • Proteins