Diagnostic procedures for paraffin-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta; Maria Laura De Marchis; Giorgia Ludovici; Patrizia Ferroni; Pasquale Abete; Fiorella Guadagni; David Della-Morte

doi:10.1007/978-1-4939-0956-8_4

Diagnostic procedures for paraffin-embedded tissues analysis in pharmacogenomic studies

Methods Mol Biol. 2014:1175:45-65. doi: 10.1007/978-1-4939-0956-8_4.

Authors

Raffaele Palmirotta¹, Maria Laura De Marchis, Giorgia Ludovici, Patrizia Ferroni, Pasquale Abete, Fiorella Guadagni, David Della-Morte

Affiliation

¹ Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy.

PMID: 25150866
DOI: 10.1007/978-1-4939-0956-8_4

Abstract

In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such as K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffin-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffin-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Mutational Analysis / methods
Histocytological Preparation Techniques*
Humans
Molecular Diagnostic Techniques*
Neoplasms / drug therapy
Neoplasms / genetics
Pharmacogenetics / methods*
Precision Medicine