Abstract
During neuroinflammatory or neurodegenerative diseases, it is often critical to characterize the composition of infiltrating immune cells. This protocol describes a reliable, fast, and simple method for the isolation of leukocytes from murine central nervous system (CNS) during steady state or inflammation for analysis by flow cytometry or other techniques.
MeSH terms
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Animals
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Cell Separation / methods
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Central Nervous System / cytology*
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Central Nervous System / immunology*
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Dendritic Cells / cytology
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Dendritic Cells / pathology
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Encephalitis / immunology
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Encephalitis / pathology
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Leukocytes / cytology*
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Leukocytes / pathology
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Lymphocytes / pathology
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Macrophages / cytology
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Macrophages / pathology
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Mice
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Microglia / cytology
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Microglia / pathology
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Monocytes / pathology
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Neutrophils / pathology