Abstract
Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer / methods
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Animals
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Cells, Cultured
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Gene Transfer Techniques*
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Hematopoiesis / genetics
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Hematopoietic Stem Cell Transplantation / methods
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / metabolism
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Immunity, Cellular / genetics*
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Immunity, Cellular / immunology
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Mice
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Mice, Transgenic
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Receptors, Antigen, T-Cell / genetics*
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Retroviridae / immunology
Substances
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Receptors, Antigen, T-Cell