Preparation and cytotoxicity of N,N,N-trimethyl chitosan/alginate beads containing gold nanoparticles

Int J Biol Macromol. 2015 Jan:72:466-71. doi: 10.1016/j.ijbiomac.2014.08.020. Epub 2014 Aug 23.

Abstract

Polyelectrolyte complex beads based on N,N,N-trimethyl chitosan (TMC) and sodium alginate (ALG) were obtained. This biomaterial was characterised by FTIR, TGA/DTG, DSC and SEM analysis. The good properties of polyelectrolyte complex hydrogel beads were associated, for the first time, with gold nanoparticles (AuNPs). Through a straightforward methodology, AuNPs were encapsulated into the beads. The in vitro cytotoxicity assays on the Caco-2 colon cancer cells and healthy VERO cells showed that the beads presented good biocompatibility on both cell lines, whereas the beads loaded with gold nanoparticles (beads/AuNPs) was slightly cytotoxic on the Caco-2 and VERO cells.

Keywords: Alginate; Beads; Gold nanoparticles; N,N,N-trimethyl chitosan; Polyelectrolyte complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / administration & dosage
  • Alginates / chemistry*
  • Animals
  • Caco-2 Cells
  • Chitosan / administration & dosage
  • Chitosan / adverse effects
  • Chitosan / chemistry*
  • Chlorocebus aethiops
  • Drug Carriers / administration & dosage
  • Drug Carriers / chemistry*
  • Glucuronic Acid / administration & dosage
  • Glucuronic Acid / chemistry
  • Gold / chemistry
  • Hexuronic Acids / administration & dosage
  • Hexuronic Acids / chemistry
  • Humans
  • Metal Nanoparticles / administration & dosage
  • Metal Nanoparticles / chemistry*
  • Vero Cells

Substances

  • Alginates
  • Drug Carriers
  • Hexuronic Acids
  • Gold
  • Glucuronic Acid
  • Chitosan