Abstract
Colon cancer is the second most common cause of cancer mortality in the Western world with metastasis commonly present at the time of diagnosis. Screening for propagation and metastatic behavior in a novel chimeric-mouse colon cancer model, driven by mutant p53 and β-Catenin, led to the identification of a unique, invasive adenocarcinoma. Comparison of the genome of this tumor, CB42, with genomes from non-propagating tumors by array CGH and sequencing revealed an amplicon on chromosome five containing CDK6 and CDK14, and a KRAS mutation, respectively. Single agent small molecule inhibition of either CDK6 or MEK, a kinase downstream of KRAS, led to tumor growth inhibition in vivo whereas combination therapy not only led to regression of the subcutaneous tumors, but also near complete inhibition of lung metastasis; thus, genomic analysis of this tumor led to effective, individualized treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma* / genetics
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Adenocarcinoma* / metabolism
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Adenocarcinoma* / pathology
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Adenocarcinoma* / therapy
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Animals
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Colonic Neoplasms* / genetics
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Colonic Neoplasms* / metabolism
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Colonic Neoplasms* / pathology
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Colonic Neoplasms* / therapy
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Lung Neoplasms* / genetics
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Lung Neoplasms* / metabolism
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Lung Neoplasms* / pathology
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Lung Neoplasms* / secondary
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Mice
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Mutation*
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Neoplasm Metastasis
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Neoplasm Proteins* / genetics
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Neoplasm Proteins* / metabolism
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Neoplasms, Experimental* / genetics
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Neoplasms, Experimental* / metabolism
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Neoplasms, Experimental* / therapy
Grants and funding
This work was entirely funded by AVEO Pharmaceuticals. AVEO Pharmaceuticals is/was the employer of all the authors except for Marcus Bosenberg. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.