Acceleration of antimicrobial susceptibility testing of positive blood cultures by inoculation of Vitek 2 cards with briefly incubated solid medium cultures

Evgeny A Idelevich; Isabel Schüle; Barbara Grünastel; Jörg Wüllenweber; Georg Peters; Karsten Becker

doi:10.1128/JCM.02400-14

Acceleration of antimicrobial susceptibility testing of positive blood cultures by inoculation of Vitek 2 cards with briefly incubated solid medium cultures

J Clin Microbiol. 2014 Nov;52(11):4058-62. doi: 10.1128/JCM.02400-14. Epub 2014 Aug 27.

Authors

Evgeny A Idelevich¹, Isabel Schüle¹, Barbara Grünastel¹, Jörg Wüllenweber¹, Georg Peters¹, Karsten Becker²

Affiliations

¹ Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
² Institute of Medical Microbiology, University Hospital Münster, Münster, Germany [email protected].

Abstract

Briefly incubated agar cultures from positive blood cultures were used for antimicrobial susceptibility testing (AST) by Vitek 2. The cultivation time until inoculation was 3.8 h for Gram-positive cocci and 2.4 h for Gram-negative rods. The error rates were low, providing early and reliable AST without additional time or cost expenditure.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteremia / microbiology*
Blood / microbiology*
Gram-Negative Bacteria / drug effects*
Gram-Negative Bacteria / isolation & purification
Gram-Positive Bacteria / drug effects*
Gram-Positive Bacteria / isolation & purification
Humans
Microbial Sensitivity Tests / methods*
Specimen Handling / methods*
Time Factors

Substances

Anti-Bacterial Agents